Anesthesia Surgery System

Anesthesia Surgery System

Safely deliver Anesthesia in a rigid and secure rodent surgery system

This is a remix of the 3D printed rodent stereotaxic device as developed by Lex Kravitz, which is a remix of the Mouse Head Holder as designed by John Everett Martin.

This remix’s features include

  • Stability improvements
  • Replacement of plastic threading with nuts
  • Elimination of need for tubing connectors by printing the t-barb parts
  • Allowing for breadboard fixation (imperial and metric)
  • Allowing for holding various sizes of tubing
  • Removal of the secondary air exhaust (eases printing)
  • A recess for physical monitoring (ex: temperature sensor)

Thumbscrews are nice, but expensive, so simply print out a tab and attach it to inexpensive screws.

Parts and Print Settings

Although not absolutely necessary, the bitebar, earbar, and mask are all parts that can benefit from higher resolution printing. We therefore recommend printing these in microSLA. If you do print these parts in FDM, don’t print in ABS (for reasons detailed here). Also, there may be some concern of the parts not being air-tight. For this, you could use the biosafe wood glue (as detailed here).

All other parts can be printed on a basic FDM printer. We wanted a very stable setup, so we printed in carbon infused filament with excellent results.

Don’t have such a 3D printer or the best material? Neither do we. We use Rosenberg Industries. We have had great success with their help, and they are aware of ONE Core projects and requirements.

All threading can be provided with a basic M3 Nut (McMaster 90592a085), no glue necessary. Simply drop the nut in the provided space. An M3 screw (McMaster 91292a113) can fit and holds the parts rigidly. Print out knob and simply push it onto the end of the screw to act as a thumbscrew.

Zip ties or twisty ties help secure the tubing to the base.


File Description
3DStereotax_bitebar.stl SLA.
3DStereotax_earbar.stl SLA. Print 2
M3ScrewHeadMountKnob.ipt FDM. Print 4
M3ScrewHeadMountKnob.stl FDM. Print 4
StereotaxONECoreBasev3.ipt FDM.
StereotaxONECoreBasev3.stl FDM.
StereotaxONECoreMask.v3.ipt SLA.
StereotaxONECoreMask.v3.stl SLA.
StereotaxONECoreSliderv3.ipt SLA.
StereotaxONECoreSliderv3.stl SLA.

Note: you can view the file in your browser by clicking on the .stl link. You can then download the file with the down arrow box button. Editable .ipt files download directly with the ipt link.


The base prints with holes like Swiss cheese. This allows the part to offer multiple ways to secure the base to a breadboard. All holes in line with semicircular indents on the part’s edges will screw into an Imperial breadboard. All other holes align to a metric breadboard. Tubing can be secured to the base in two locations by dropping the tubing in the holders and tying them down with zip or twisty ties. There is a semicircular cutout which allows for placement of monitoring equipment (temperature) under a heating pad (Arduino controlled system coming soon!). If you don’t plan on using this, you can simply fill this cutout with the associated fill in spacer that will print with the base.

1.png 2.png

ONE Core acknowledgement

Please acknowledge the ONE Core facility in your publications. An appropriate wording would be:

“The Optogenetics and Neural Engineering (ONE) Core at the University of Colorado School of Medicine provided engineering support for this research. The ONE Core is part of the NeuroTechnology Center, funded in part by the School of Medicine and by the National Institute of Neurological Disorders and Stroke of the National Institutes of Health under award number P30NS048154.”