Low Profile Diamond Bath Imaging Chamber
Projects | | Links:
An open-source low profile diamond bath imaging chamber
Use this chamber to hold your cells, tissues, or slices while doing your cellular recordings (intracellular, extracellular, the possibilities!), imaging (upright, inverted, whatever!), or patch clamping. The low profile allows for ease of electrode access and the shape encourages laminar flow from the inlet to the outlet. The shape fits typical cover slips (22 x 40 mm) and platforms.
It is STRONGLY recommended to print the part in a biosafe material. This means no ABS. Nylon may be tempting because it is autoclavable, but it has been shown to hyperpolarize neurons (see The use of three-dimensional printing to produce in vitro slice chambers, Hyde. J et al. 2014. Also, check out out 3D printing rambling
Need to control temperature of your slices? Check out our Temperature Controlled Slice Holder System! Doing patch clamp experiments? Harness the power of the amazing Dynamic Clamp project)!
| File | Description | File Type |
|---|---|---|
| electrophysiology_chamber.ipt | Stereotaxic Optic Fiber Holder | ipt (3D editable) |
| electrophysiology_chamber.stl | Stereotaxic Optic Fiber Holder | stl (3D printable) |
If interact-able views of the 3D parts do not load, try reloading the page. If that doesn’t work, view them on the GitLab pages (links above) or update your browser.
ONE Core acknowledgement
Please acknowledge the ONE Core facility in your publications. An appropriate wording would be:
“The Optogenetics and Neural Engineering (ONE) Core at the University of Colorado School of Medicine provided engineering support for this research. The ONE Core is part of the NeuroTechnology Center, funded in part by the School of Medicine and by the National Institute of Neurological Disorders and Stroke of the National Institutes of Health under award number P30NS048154.”
