Low Profile Diamond Bath Imaging Chamber

Low Profile Diamond Bath Imaging Chamber

An open-source low profile diamond bath imaging chamber

Use this chamber to hold your cells, tissues, or slices while doing your cellular recordings (intracellular, extracellular, the possibilities!), imaging (upright, inverted, whatever!), or patch clamping. The low profile allows for ease of electrode access and the shape encourages laminar flow from the inlet to the outlet. The shape fits typical cover slips (22 x 40 mm) and platforms.

It is STRONGLY recommended to print the part in a biosafe material. This means no ABS. Nylon may be tempting because it is autoclavable, but it has been shown to hyperpolarize neurons (see The use of three-dimensional printing to produce in vitro slice chambers, Hyde. J et al. 2014. Also, check out out 3D printing rambling

Need to control temperature of your slices? Check out our Temperature Controlled Slice Holder System! Doing patch clamp experiments? Harness the power of the amazing Dynamic Clamp project)!

File Description File Type
electrophysiology_chamber.ipt Stereotaxic Optic Fiber Holder ipt (3D editable)
electrophysiology_chamber.stl Stereotaxic Optic Fiber Holder stl (3D printable)

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ONE Core acknowledgement

Please acknowledge the ONE Core facility in your publications. An appropriate wording would be:

“The Optogenetics and Neural Engineering (ONE) Core at the University of Colorado School of Medicine provided engineering support for this research. The ONE Core is part of the NeuroTechnology Center, funded in part by the School of Medicine and by the National Institute of Neurological Disorders and Stroke of the National Institutes of Health under award number P30NS048154.”